1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Aminohydantion (AHD) in the tissue(chicken, fish shrimp). The enzyme conjugate is pre-coated on the micro-well stripes. The Aminohydantion (AHD) in the testing sample competes with the conjugate antigen pre-coated on the micro-well stripes, to interact with the antibody against AHD. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the testing sample has a negative correlation with the content of AHD in it. This value is compared to the standard curve and the content of the corresponding AHD is subsequently obtained.

2. Technical specifications
Sensitivity : 0.1ppb
Detection limit:
Tissue (some nterference in shrimp and fish) ——— 0.3ppb
Tissue and honey—————————--0.2ppb
Recovery rate
Tissue (shrimp and fish) ————90±15%
Honey ,chicken meat /liver——— 80±15%
Cross-reaction rate:
AHD ——————100%
AMOZ————— ﹤0.1%
AOZ ————— ﹤0.1%
SEM————— ﹤0.1%